Journal: bioRxiv

Article Title: Computationally guided design of a metastasis-on-a-chip platform for quantitative evaluation of chemotactic cues in developmental cancers

doi: 10.64898/2026.07.25.740695

Figure Lengend Snippet: (A) Expression of KDR, encoding VEGFR2, and (B) FLT4, encoding VEGFR3, across publicly available pediatric solid tumor datasets included in the R2 MegaSampler platform. Expression values are presented as log2-transformed signal intensity. The distributions illustrate inter-dataset and inter-tumor heterogeneity in the expression of receptors associated predominantly with VEGF-A and VEGF-C signaling, respectively. M, metastasis; T, primary tumor; ES, Ewing sarcoma; NB, neuroblastoma; OS, osteosarcoma.

Article Snippet: VEGF-A165 and VEGF-C concentrations were quantified using commercially available human ELISA kits (RayBiotech, Peachtree Corners, GA, USA; Catalog Nos.

Techniques: Expressing, Transformation Assay

Journal: bioRxiv

Article Title: Computationally guided design of a metastasis-on-a-chip platform for quantitative evaluation of chemotactic cues in developmental cancers

doi: 10.64898/2026.07.25.740695

Figure Lengend Snippet: VEGF-C transport was simulated using initial concentrations of 0.1 or 5 µg/mL in Chamber 2. Predicted VEGF-C concentrations over time are shown in (A) Channel 2, adjacent to the source, (B) at the microchannel array separating Reservoirs 2 and 1, and (C) in Channel 1, on the Chamber 1 side of the device. An initial concentration of 5 µg/mL generated measurable exposure at the microchannel array within the experimental time window, whereas 0.1 µg/mL produced substantially lower concentrations and negligible predicted exposure at the microchannel array and in Channel 1. Different concentration units and y-axis ranges were used for the individual device regions.

Article Snippet: VEGF-A165 and VEGF-C concentrations were quantified using commercially available human ELISA kits (RayBiotech, Peachtree Corners, GA, USA; Catalog Nos.

Techniques: Concentration Assay, Generated, Produced

Journal: bioRxiv

Article Title: Computationally guided design of a metastasis-on-a-chip platform for quantitative evaluation of chemotactic cues in developmental cancers

doi: 10.64898/2026.07.25.740695

Figure Lengend Snippet:

Article Snippet: VEGF-A165 and VEGF-C concentrations were quantified using commercially available human ELISA kits (RayBiotech, Peachtree Corners, GA, USA; Catalog Nos.

Techniques: Incubation, Cell Culture, Enzyme-linked Immunosorbent Assay

Journal: bioRxiv

Article Title: Computationally guided design of a metastasis-on-a-chip platform for quantitative evaluation of chemotactic cues in developmental cancers

doi: 10.64898/2026.07.25.740695

Figure Lengend Snippet: (A) Representative merged bright-field and fluorescence images of neuroblastoma, Ewing sarcoma, and osteosarcoma cells detected within the microchannel array under control, VEGF-A165, and VEGF-C conditions. Images were processed using a custom Fiji macro that automatically identified the microchannel region, detected fluorescent cells, and retained cells located within the microchannels according to predefined size, circularity, intensity, and channel-overlap criteria. Scale bars =50µm. (B) Quantification of the number of cells detected within the microchannels for neuroblastoma, Ewing sarcoma, and osteosarcoma. Individual data points represent independent microfluidic devices, and bars show mean ± SD. Statistical significance was assessed using ordinary one-way ANOVA followed by Dunnett’s multiple-comparisons test against the corresponding control. *p < 0.05; ns, not significant.

Article Snippet: VEGF-A165 and VEGF-C concentrations were quantified using commercially available human ELISA kits (RayBiotech, Peachtree Corners, GA, USA; Catalog Nos.

Techniques: Fluorescence, Control

Journal: Journal of Translational Medicine

Article Title: Single-cell and spatial transcriptomic analysis reveals PAX5’s role and regulatory mechanisms in gastric adenocarcinoma lymph node metastasis

doi: 10.1186/s12967-026-08059-4

Figure Lengend Snippet: PAX5 suppresses invasion and migration of GAC lymph node metastatic cells. A : CCK-8 assay measuring the viability of GAC lymph node metastatic cells. B and E: Scratch assay demonstrating cell migration; scale bar = 100 μm. C and F: Transwell assay assessing cell invasion; scale bar = 100 μm. D and F : Immunofluorescence analysis of PAX5 expression; scale bar = 50 μm. G : Quantitative PCR (qPCR) analysis of mRNA expression levels of PAX5, VEGF-C, PIK3, and AKT1. H : Western blot analysis of protein expression levels of PAX5, VEGF-C, AKT1, PI3K, MMP2, and Ki-67. “ns” indicates “not significant”; * indicates P < 0.05; ** indicates P < 0.01; *** indicates P < 0.001; **** indicates P < 0.0001

Article Snippet: Antibody details are as follows: PAX5 (A12048, ABclonal, Wuhan, China), VEGF-C (A2556, ABclonal, Wuhan, China), AKT1 (A17909, ABclonal, Wuhan, China), PI3K (A19742, ABclonal, Wuhan, China), MMP2 (A19080, ABclonal, Wuhan, China), and Ki-67 (A16919, ABclonal, Wuhan, China).

Techniques: Migration, CCK-8 Assay, Wound Healing Assay, Transwell Assay, Immunofluorescence, Expressing, Real-time Polymerase Chain Reaction, Western Blot

Journal: Journal of Translational Medicine

Article Title: Single-cell and spatial transcriptomic analysis reveals PAX5’s role and regulatory mechanisms in gastric adenocarcinoma lymph node metastasis

doi: 10.1186/s12967-026-08059-4

Figure Lengend Snippet: PAX5 modulates HLEC cell tube formation in a co-culture system. A : Quantification of the number of nodes and total length of major master segments in tube-like structures. B : qPCR analysis of mRNA expression levels of PAX5, VEGF-C, PIK3, and AKT1 in HLEC cells. “ns” indicates “not significant”; * indicates P < 0.05; ** indicates P < 0.01; *** indicates P < 0.001; **** indicates P < 0.0001; scale bars = 100 μm

Article Snippet: Antibody details are as follows: PAX5 (A12048, ABclonal, Wuhan, China), VEGF-C (A2556, ABclonal, Wuhan, China), AKT1 (A17909, ABclonal, Wuhan, China), PI3K (A19742, ABclonal, Wuhan, China), MMP2 (A19080, ABclonal, Wuhan, China), and Ki-67 (A16919, ABclonal, Wuhan, China).

Techniques: Co-Culture Assay, Expressing

Journal: Journal of Translational Medicine

Article Title: Single-cell and spatial transcriptomic analysis reveals PAX5’s role and regulatory mechanisms in gastric adenocarcinoma lymph node metastasis

doi: 10.1186/s12967-026-08059-4

Figure Lengend Snippet: In vivo validation of PAX5 functional mechanisms. A : Hematoxylin and eosin (H&E) staining of mouse tumors. B : Immunohistochemical analysis of PAX5, Ki-67, and MMP2 expression in mouse tumors. C : qPCR analysis of mRNA expression levels of PAX5, PIK3, AKT1, and VEGF-C in mouse tumors. D : Western blot analysis of protein expression levels of PAX5, PI3K, AKT1, and VEGF-C in mouse tumors. * indicates P < 0.05; ** indicates P < 0.01; *** indicates P < 0.001; **** indicates P < 0.0001; scale bars = 50 μm

Article Snippet: Antibody details are as follows: PAX5 (A12048, ABclonal, Wuhan, China), VEGF-C (A2556, ABclonal, Wuhan, China), AKT1 (A17909, ABclonal, Wuhan, China), PI3K (A19742, ABclonal, Wuhan, China), MMP2 (A19080, ABclonal, Wuhan, China), and Ki-67 (A16919, ABclonal, Wuhan, China).

Techniques: In Vivo, Biomarker Discovery, Functional Assay, Staining, Immunohistochemical staining, Expressing, Western Blot